This semester I will focus on helping Odalys, a graduate student who is working with my mentor, Dr. Carlos Rodriguez. She needs lots of help on this phase of her work which involves the construction of an E. coli clone’s library. These clones have a recombinant plasmid with inserts of PCR products resulting from gene amplification of antibiotic resistance and integrases (they capture these resistance genes and incorporate them into a factory of operons known as the integrons). These PCR products were amplified from DNA samples taken from different coastal habitats exposed to different levels human influence. The idea is to evaluate if there’s a relationship between the level impact or contamination by human activity and the abundance and distribution of these genes.
Goals you plan to achieve by the end of this semester.
“By the end of this semester I plan to …
1) finish building the clones of E. coli necessary for the graduate student project Odalys.
2) continue my initial research.
3) get a rewarding experience this semester in the laboratory.
Last semester I was working with another project with Dr. Carlos Rodriguez called “Enrichment of microbes producing electricity using graphite electrodes.” However, for now (the beginning of this semester), I’ll be helping a graduate student, Odalys, in her project that has little to do with what I was doing so far. Nevertheless, I’ll put into practice the basic techniques of microbiology, which will be very useful when continuing with the initial project.
I hope later in the course of the semester to continue the initial project assigned by my mentor in this way I’ll develop new techniques (since I had never worked with biofilms). By far I’ll only review basic techniques of microbiology by helping Odalys graduate student, who can guide me to make these techniques in a less theoretical and more practically effective way.